[ Mexicaanse griep is gevaarlijk ]

Fret en cellijn tonen risicoprofiel H1N1-virus

6 juli 2009 Tim Snoek 25 reacties 1046x gelezen

Het Mexicaanse griepvirus is gevaarlijker dan een normaal seizoensgriepvirus. Onderzoekers van het Erasmus Medisch Centrum in Rotterdam concluderen dit aan de hand van een studie in fretten, die zij publiceerden in Science.

De onderzoekers isoleerden het Mexicaanse griepvirus uit een neusslijmvliesmonster van het eerste Mexicaanse griepgeval in Nederland. Vervolgens infecteerden ze zes fretten met dit virus, zes andere fretten kregen een seizoensgriepvirus. Van alle fretten werden dagelijks monsters uit de keel en neus genomen.

Het aantal virusdeeltjes in de monsters werd bepaald met end-point titratie van de cellijn MDCK, waarbij je het aantal cellen bepaalt dat geïnfecteerd raakt door verschillende verdunningen van het oorspronkelijke monster. Het bleek dat de dieren die met het Mexicaans griepvirus besmet waren, anderhalf keer meer virusdeeltjes uit de keel uitscheidden dan de fretten die een seizoensgriepvirus onder de leden hadden.

Ook werden enkele besmette fretten opgeofferd. Met immunohistochemie analyseerden de onderzoekers welke weefsels met virus geïnfecteerd waren. Het Mexicaanse griepvirus bleek tot in de onderste luchtwegen door te dringen, in tegenstelling tot het seizoensgriepvirus dat niet verder komt dan de bovenste luchtwegen. Dit maakt de kans op een longontsteking een stuk groter.

Bron: Science

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